|
KMID : 1123920130270060809
|
|
Korean Journal of Oriental Physiology and Pathology
2013 Volume.27 No. 6 p.809 ~ p.817
|
|
|
Effect of Lespedezea Cuneata on the Contraction of Rabbit Common Carotid Artery and Corpus Cavernosum
|
|
Park Sun-Young
Kim Ho-Hyun
|
|
|
Abstract
|
|
|
|
The aim of this study was to evaluate the mechanism of vasodilation of Lespedezea cuneata(LC) in rabbit common carotid artery and cavernosal smooth muscle. LC relaxed arterial strips precontracted with norepinephrine and cavernosal strips precontracted with phenylephrine. The arterial relaxation effects of LC was endothelium-dependent. Nω-nitro-L-arginine(L-NNA), NOS inhibitor, methylene blue(MB), cGMP inhibitor, indomethacin(IM), cyclo-oxygenase inhibitor and tetraethylammonium chloride(TEA), KCa-channel blocker attenuate the relaxation responses of LC in arterial strips. In Ca2+-free krebs-ringer solution, pretreatment of LC extract significantly reduced the contraction induced by addition Ca2+. L-NNA reduced LC extract-induced relaxation in cavernosal strips, but IM, TEA and MB didn't affect LC extract-induced relaxation. When LC extract was applicated on human umbilical vein endothelial cell, the nitric oxide concentration was increased. We conclude that in rabbit common carotid artery, LC may suppress influx of extra-cellular Ca2+ through the release of endothelium derived relaxing factor including nitric oxide, prostacyclin, endothelium derived hyperpolarizing factor. And LC exerts a relaxing effect on corpus cavernosum through activating the NO.
|
|
|
KEYWORD
|
|
|
Lespedezea cuneata, nitric oxide, corpus cavernosum, arterial relaxation
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|